Innovative Preclinical Research for Bovine Papular Stomatitis Virus Drug Development & Diagnostic Solution

Overview

The Parapoxvirus (PPV) family includes Bovine Papular Stomatitis Virus (BPSV), which mainly infects cattle and targets calves specifically. The disease manifests as papules and mild erosions on both the oral mucosa and udders, while reinfection happens frequently because prior infections fail to produce strong immunity. The virus transmits to milkers, farmers, and veterinarians through contact or indirect means, causing skin lesions like foot-and-mouth disease. Timely and precise diagnosis remains critical to control BPSV because it displays similar clinical signs to other vesicular diseases. The lengthy and less sensitive nature of traditional diagnostic methods, such as virus isolation and histopathology, has led researchers to create molecular diagnostic techniques, including PCR, qPCR, and LAMP assays, which deliver better sensitivity and cost-effectiveness. Creative Biolabs delivers full-spectrum preclinical research capabilities to promote progress in BPSV research and treatment development. Our services include preclinical efficacy testing with animal models to determine both the safety and effectiveness of BPSV therapeutics. We combine our specialized knowledge and advanced facilities to speed up the creation of vaccines and therapies for BPSV while supporting your research success.

Accelerated BPSV Preclinical Research Services (Therapeutics & Diagnostics)

• For Therapeutics Development

In Vitro Antiviral Efficacy Assays

• Cytopathic Effect (CPE) Inhibition Assay: Measures the compound's ability to prevent virus-induced damage to host cells.
• Plaque Reduction Assay (PRA): Quantifies reduction in virus plaque formation in the presence of a drug.
• Virus Yield Reduction Assay: Measures decrease in viral titers (e.g., TCID₅₀ or qPCR quantification of viral genome) after treatment.
• Time-of-Addition Studies: Pinpoint the stage in the virus life cycle affected by the drug (entry, replication, assembly, release).
• Mechanism-of-Action Studies: Investigate how the drug interacts with specific viral or host cell targets

In Vivo Efficacy Studies (Animal Models)

• BPSV Challenge Models in Calves or Surrogate Hosts: Typically use young calves, as they are natural hosts.
• Therapeutic Dosing Studies: Test multiple doses and administration routes (oral, intranasal, etc.).
• Toxicology & Safety Assessment: Monitor for systemic toxicity, weight loss, blood biomarkers, and organ histopathology.
• Pharmacokinetics (PK) and Pharmacodynamics (PD): Measure absorption, distribution, metabolism, and excretion (ADME).

• For Diagnostics Development

Antigen Detection Assays

• Antigen-Capture ELISA: Uses monoclonal or polyclonal antibodies to capture viral antigens in samples (e.g., lesion swabs, vesicle fluid).
• Lateral Flow Assays (LFA) / Rapid Antigen Tests: Immunochromatographic strips.
• Immunofluorescence Assay (IFA): Uses fluorescent antibodies to detect viral antigens in infected tissue or cells.
• Immunohistochemistry (IHC): Detects antigens in tissue sections.

Nucleic Acid Detection Assays

• Conventional PCR: Detects conserved viral genes (e.g., B2L gene).
• Real-Time PCR (qPCR): Quantifies viral DNA in real-time.
• Loop-Mediated Isothermal Amplification (LAMP): Isothermal reaction (no thermocycler required).
• Recombinase Polymerase Amplification (RPA): Like LAMP, but often faster (10–20 minutes).
• Multiplex PCR: Differentiates BPSV from other parapoxviruses (e.g., Orf virus, pseudocowpox).

Antibody Detection Assays (Serology)

• Indirect ELISA: Detects BPSV-specific antibodies in serum or plasma.
• Virus Neutralization Test (VNT): Functional assay measuring the ability of antibodies to neutralize the virus.
• Western Blot: Confirms antibody response to specific viral proteins.

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Products for BPSV Research

Recombinant proteins function as lab-produced BPSV antigens, which serve diagnostic purposes and support research and vaccine development. Monoclonal antibodies attach to BPSV proteins with high specificity, which allows accurate detection through ELISA and IHC assays. They enable researchers to perform precise, sensitive, and dependable immunological studies and BPSV diagnosis through their combined use.

• Recombinant BPSV proteins or antigens

• Antibodies against BPSV proteins

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Applications

1. Early Detection and Outbreak Control: PCR detects active BPSV infections at an early stage, which enables veterinarians to quarantine infected animals while stopping virus transmission in herds.

2. Epidemiological Surveillance: Researchers utilize serological assays such as ELISA to monitor herd immunity status and virus prevalence, which produces essential information needed for effective disease control.

3. Differentiation from Other Diseases: Diagnostic methods enable the distinction between BPSV and other similar diseases, such as foot-and-mouth disease, to ensure correct diagnosis and treatment.

Advantages

1. Accurate and Reliable Testing: PCR and ELISA represent advanced diagnostic tools that provide exact BPSV detection, enabling clients to make informed decisions.

2. Early Disease Detection: Quick identification of infections enables faster response actions, which reduce the likelihood of herd outbreaks.

3. Comprehensive Solutions: The company offers multiple diagnostic services such as PCR testing, antigen detection, and serological assays, which together support the full management of BPSV.

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