Specialized Preclinical Research Service for Shope Fibroma Virus (Rabbit Fibroma) Treatment Development & Diagnostic Method

Overview

Rabbit Fibroma Virus belongs to the Poxviridae family under the genus Leporipoxvirus and is scientifically known as Shope Fibroma Virus (SFV). This virus infects rabbits to produce benign skin tumors, which naturally regress over time. Richard Shope discovered this virus in the 1930s, and researchers use it to study tumor development and immune response because the virus produces tumors. The Shope Fibroma Virus shares a close relation to Myxoma virus while advancing scientific knowledge about viral pathogenesis and tumor biology. The virus spreads through arthropod carriers, including mosquitoes and ticks, while healthy animals usually recover without medical intervention. SFV continues to serve as an essential research tool in virology because scientists have thoroughly characterized its life cycle and host-specific behavior. Creative Biolabs provides complete preclinical research solutions for Shope Fibroma Virus, which includes in vivo rabbit models along with viral propagation techniques combined with immune response assessment and histopathological evaluation methods. We provide support services for antiviral development research and tumor immunology investigations.

Accelerated Shope Fibroma Virus (Rabbit Fibroma) Preclinical Research Services

• For Therapeutics Development

In Vitro Antiviral Efficacy Assays

• Cytotoxicity Assays (e.g., MTT, LDH, or ATP assays): To determine the toxicity of the therapeutic candidates to host cells.
• Viral Inhibition Assay: This tests the ability of a drug or compound to inhibit viral replication. It could involve assessing the viral load reduction or changes in viral protein expression.
• Cell-Based Assays: Often involves the use of cultured cells infected with SFV to assess the antiviral activity of the therapeutic candidate, including quantifying the decrease in viral titer or viral genome copies.
• Time-of-Addition Assay: This assesses the impact of the drug at different stages of viral replication (entry, replication, assembly, or release).

In Vivo Efficacy Studies (Animal Models)

• Animal Model Selection: Depending on the therapeutic candidate, appropriate animal models such as mice, rabbits, or non-human primates are chosen.
• Dosing and Administration: The route of administration (oral, intravenous, intraperitoneal, etc.), frequency, and duration of treatment are studied to determine the optimal therapeutic regimen.
• Efficacy Monitoring: This includes measuring viral load in tissues, assessing survival rates, monitoring clinical symptoms (e.g., tumor growth, skin lesions), and evaluating overall health.
• Pharmacokinetics (PK) and Pharmacodynamics (PD): These studies determine how the drug is absorbed, distributed, metabolized, and eliminated in the animal, and the relationship between the drug concentration and its effect on the virus or tumor.

• For Diagnostics Development

Antigen Detection Assays

• Western Blotting: This method is used to identify specific SFV proteins by separating viral proteins based on size using SDS-PAGE, followed by transfer to a membrane and detection with specific antibodies.
• Immunohistochemistry (IHC): IHC is used to detect SFV antigens in tissue sections, providing spatial localization of the virus within tissues, such as in skin lesions or tumor sites.
• Flow Cytometry: This can be used for the detection of SFV antigens on the surface of infected cells, especially in a mixed population of cells.

Nucleic Acid Detection Assays

• Southern Blotting: For the detection of viral DNA in tissues or cells, Southern blotting allows for the identification of specific SFV genome sequences after gel electrophoresis and membrane transfer.
• Next-Generation Sequencing (NGS): NGS can be used to analyze viral genomes in high resolution, allowing for detailed analysis of the viral strains, mutations, or resistance development in each population.
• In Situ Hybridization (ISH): This is used to detect SFV-specific RNA in tissue samples, providing localization information about viral replication sites within the host.

Antibody Detection Assays (Serology)

• Western Blotting: Used to confirm the presence of specific antibodies against SFV by identifying the bands corresponding to viral proteins.
• Indirect Immunofluorescence Assay (IFA): In this assay, SFV-infected cells are fixed on a slide and incubated with serum samples from the host.
• Neutralization Assay: This assay tests the ability of antibodies in a host serum to neutralize the infectivity of SFV.

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Products for Shope Fibroma Virus (Rabbit Fibroma) Research

SFV recombinant proteins are generated through specialized expression systems. These proteins contain essential viral antigens that facilitate diagnostics and vaccine development as well as immunological research. These proteins function as tools to precisely identify the responses of the host's immune system. The high purity and stability of these proteins make them perfect for both laboratory and preclinical research applications.

• Recombinant Shope Fibroma Virus (Rabbit Fibroma) proteins or antigens

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Applications

• Researchers use SFV as a foundational model to explore both poxvirus biology and how viruses cause tumors.
• Research applications of SFV include examination of host immune reactions to viral infections and the process of tumor regression. The system acts as a testing platform for antiviral drugs and immunomodulators alongside vaccine candidates.
• The relatedness of Shope Fibroma Virus to other leporipoxviruses including Myxoma virus provides significant benefits for comparative virology research and gene therapy vector development.

Advantages

1. Comprehensive Capabilities: Our support services extend from in vitro assays to in vivo models and cover diagnostic assay development and therapeutic evaluation.

2. Experienced Team: Our scientific team holds deep knowledge in virology combined with immunology and preclinical research capabilities.

3. Customized Solutions: Our services adapt to fulfill research objectives across all stages from initial discovery to preclinical validation.

4. High-Quality Standards: Every study maintains rigorous quality control and regulatory compliance standards to guarantee dependable and repeatable findings.

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