We DO NOT PROVIDE ANY PRODUCTS OR SERVICES DIRECTLY TO PATIENTS. All of our products are for Research Use Only (RUO), NOT intended for diagnostic, therapeutic, or clinical use.
Creative Biolabs offers expert services for the development of antibodies targeting the F13L protein of monkeypox virus (MPXV), a vital component in understanding the pathogenesis of the virus and its immune evasion mechanisms. The F13L gene of monkeypox encodes a protein that plays a crucial role in the virus's ability to inhibit host immune responses, particularly the antiviral activity of interferons. As MPXV continues to be a global health concern, antibody-based research tools against this protein have become pivotal for studying viral replication, immune modulation, and potential vaccine development.
Creative Biolabs provides a comprehensive range of services to support the development of specific antibodies against the F13L protein. Our approach includes custom monoclonal and polyclonal antibody production, with each stage designed to ensure high specificity, affinity, and functionality in subsequent assays.
We employ cutting-edge hybridoma technology to produce high-affinity monoclonal antibodies specific to the F13L protein. The process begins with immunizing selected animals (typically mice or rabbits) with purified F13L protein or its peptides. This immunization induces the production of antibodies, which are then harvested and screened for specificity and binding affinity. The highest-performing clones are selected for further development and mass production.
We offer polyclonal antibody development services for researchers who require antibodies with a broader recognition spectrum of the F13L protein. This service utilizes a similar immunization process, but antibodies from multiple animal sources are pooled to create a diverse antibody population, ensuring a more comprehensive immune response.
For both monoclonal and polyclonal antibodies, we provide extensive characterization services to assess the specificity, sensitivity, and functional activity of the antibodies. This includes techniques such as ELISA, Western blotting, and IHC. The antibodies are validated for binding specificity to the F13L protein, cross-reactivity with related viral proteins, and their potential for neutralization assays.
To facilitate various experimental applications, Creative Biolabs offers conjugation and labeling services for F13L antibodies. This includes the attachment of fluorescent dyes, biotin, or enzymes to the antibodies, enabling their use in imaging, detection, and diagnostic assays.
Understanding the specific regions of the F13L protein that elicit immune responses is crucial for both vaccine and therapeutic development. Our epitope mapping services help identify and characterize the antigenic sites recognized by the F13L antibodies, providing valuable insights for vaccine design and other therapeutic strategies.
The F13L protein is an essential factor in the immune evasion strategies employed by the monkeypox virus. It has been shown to inhibit host interferon response, specifically by binding to the signaling molecules involved in the antiviral action of type I interferons. This activity effectively dampens the host's innate immune response, allowing the virus to replicate more efficiently in host cells.
Furthermore, F13L participates in the modulation of macrophage activity, a critical component in immune surveillance. By interfering with the activation of these immune cells, the protein contributes to the virus's ability to evade detection and immune clearance. This makes F13L an attractive target for antibody-based interventions aimed at neutralizing the virus and restoring host immune responses.
| Cat# | Product Name | Target | Antibody Isotype | Species Reactivity | Applications |
|---|---|---|---|---|---|
| MPYF-1122-KX1613 | Anti-Squirrel Parapoxvirus F13L Monoclonal Antibody (MP-K2413) (Mouse IgG) | F13L | Mouse IgG | Squirrel Parapoxvirus | WB (Other applications need to be tested) |
| MPYF-1022-KX1386 | Anti-Vaccinia Virus (Smallpox Vaccine) F13L Monoclonal Antibody (MP-K1320) (Mouse IgG) | F13L | Mouse IgG | Vaccinia Virus (Smallpox Vaccine) | WB (Other applications need to be tested) |
| Cat# | Product Name | Species | Expression System | Target Protein | Protein Length |
|---|---|---|---|---|---|
| MPYF-0922-KX521 | Magic™ Vaccinia Virus (Smallpox Vaccine) (Western Reserve) F13L Recombinant Protein | Vaccinia Virus (Smallpox Vaccine) | Based on specific requirements | F13L | Full length |
| MPYF-1022-KX749 | Magic™ Squirrel Parapoxvirus (Red squirrel UK) F13L Recombinant Protein | Squirrel Parapoxvirus | Based on specific requirements | F13L | Full length |
Creative Biolabs utilizes advanced platforms and technologies to provide high-quality antibody development services. Key technologies employed include:
For the identification of high-affinity antibody candidates.
For precise manipulation of antibody sequences.
For antibody characterization and validation.
To optimize for various detection and diagnostic assays.
F13L antibodies have broad applications in both basic research and applied science. Some of the key areas where these antibodies are utilized include:
Creative Biolabs stands out in the field of antibody development for several reasons:
Creative Biolabs offers tailored antibody production services, including choice of animal models, expression systems, and specific epitope targeting to meet your research needs for F13L antibodies.
Yes, Creative Biolabs offers scalable antibody production, from small-scale research quantities to large-scale bulk production for ongoing research applications.
Yes, Creative Biolabs provides antibody purification services using protein A/G affinity chromatography to ensure high-purity antibodies suitable for downstream applications.
Yes, Creative Biolabs provides comprehensive post-development support, including troubleshooting, assay optimization, and additional characterization to ensure the antibodies meet your specific research needs.
We DO NOT PROVIDE ANY PRODUCTS OR SERVICES DIRECTLY TO PATIENTS. All of our products are for Research Use Only (RUO), NOT intended for diagnostic, therapeutic, or clinical use.