High-Throughput Screening-Based Anti-monkeypox Drug Discovery Solutions

High-throughput screening (HTS) has been used as an efficient tool in identifying new lead compounds for antiviral drug development. It provides starting chemical matter in the adventure of developing a new drug. Creative Biolabs is an international leading CRO for anti-monkeypox drug discovery. We have established an advanced and comprehensive technology platform. Our experts have profound professional backgrounds and project experience, which can quickly promote the project.

HTS Technologies at Creative Biolabs

• Fluorescence-Based Assays

Fluorescence techniques are well suited to HTS since they can give very high sensitivity and allow fairly straightforward miniaturization. Fluorescence-based assays can be divided into two categories: cell-based assays and cell-free assays. The former usually involves assessing the activity of a target protein by monitoring the biological response of the cell in which the target protein resides, while the latter measures the biological activity of a relatively pure target.

• Nuclear Magnetic Resonance (NMR)

NMR techniques can detect interactions by observing either macromolecular or small molecular NMR parameters.

• Affinity Chromatography

Affinity chromatography is a method to study the binding of drugs or potential drugs to target molecules. It is very powerful and predictive in monitoring ligand-protein, substrate-enzyme, inhibitor-enzyme, and ligand-receptor interactions.

• Surface Plasmon Resonance (SPR) Technology

SPR is an optical technique for measuring the surface sensitivity of affinity coupling of label-free biomolecules.

• DNA Microarray

DNA microarray technology provides a unique way to examine gene expression by regularly immobilizing different kinds of DNA on a two-dimensional surface.

HTS-Based Anti-Monkeypox Drug Discovery Solutions at Creative Biolabs

• Biochemical Assays

Biochemical screens utilize purified target proteins and measure ligand binding or in vitro inhibitory enzyme activity. These assays are typically conducted in 384-well plates, which provide a good compromise between screening volumes, throughput, and the costs of more sophisticated screening equipment, and read out using an optical method such as absorbance, fluorescence, or luminescence.

• Binding-based Assays

Binding-based assays directly observe library components bound to target proteins regardless of their effect on protein function.

• Cell-based Assays

Both target-based and phenotypic cell-based approaches can lead to new drugs. Cell-based assays are used in all stages of drug discovery: target identification and validation, preliminary screening, lead identification, lead optimization, and safety and toxicological screening.

Fig.1 General classification of HTS assays. (Aldewachi, 2021)

Advantages of HTS

HTS is simple, rapid, low-cost, and efficient, based on ligand-target interaction, and can obtain higher information. HTS is a multidisciplinary field involving automated operating platforms, highly sensitive detection systems, specific screening models (in vitro), rich component libraries, and data acquisition and processing systems. Various technologies, especially new ones, are now available and it has become possible to screen more than 100,000 samples per day.

Creative Biolabs is an expert in the field of monkeypox and can provide customized services flexibly. We will work as a member of your team to find the most suitable solution to advance the project efficiently. Please feel free to contact us for more detail.