Anti-Orthopoxviruses A27 Monoclonal Antibody (MP-X9) (Mouse IgG2a, kappa)(MPYF-0822-YF9)

Target

A27

Species Reactivity

Orthopoxviruses

Cross Reactivity

Monkeypox Virus

Specificity

This antibody recognizes ECTV and some other orthopoxviruses. Its epitope is localized on a tetrapeptide located at the VACV homologue A27 (C-terminal epitope #5 (aa 68-71). Ectromelia virus (ECTV) is a virus of the family Poxviridae and the genus Orthopoxvirus that causes mousepox, a disease of mice. Mousepox causes skin lesions and generalized disease, which can be fatal. Orthopoxvirus has been associated with smallpox, cowpox, horsepox and monkeypox.

Immunogen

Sucrose-gradient purified Ectromelia virus (ECTV) Strain Munich 1

Antibody Isotype

Mouse IgG2a, kappa

Clone

MP-X9

Clonality

Monoclonal Antibody

Purification

Protein A affinity purified

Applications

Immunogold electron microscopy; IP; WB; ELISA; FC; IF

Application Notes

This antibody is recommended for the detection and analysis of Orthopox viruses, especially Ectromelia virus (ECTV). It binds to the epitope on the virus which seems not to be engaged with viral cell fusion. VACV-5B1 (5B1/2G6) is a non-neutralizing clone that can be utilized for the detection of the analog of A27 protein on Orthopoxviruses. This antibody's antigenic site is located in a functionally very important area within the C-terminus of the A27 protein; in this hydrophilic region, the two cysteines at positions 71 and 72 are responsible for formation of disulfide bonds and, therefore, play an important role for a functionally active trimeric A27 structure. Mouse IgG2a version of this clone (EM-5B1/2G6) was also used to develop an ELISA capture assay as a capture antibody and was shown to be superior in detecting Orthopox viruses in comparison with other monoclonal and polyclonal clones. It makes it a very good candidate for the development of an anti-orthopox viruse detection assay.

Buffer

PBS with 0.02% Proclin 300

Preservative

0.02% Proclin 300

Storage

Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.