Anti-Orthopoxviruses A27 Monoclonal Antibody (MP-X11) (Mouse IgG2a/Human IgG1, Kappa)(MPYF-0822-YF11)

This antibody recognizes Orthopoxviruses A27. The isotype is Mouse IgG2a/Human IgG1, Kappa. It can be used in applications: Immunogold electron microscopy; IP; WB; ELISA; FC; IF.

Product Description
Target
A27
Species Reactivity
Orthopoxviruses
Cross Reactivity
Monkeypox Virus
Specificity
This antibody recognizes ECTV and some other orthopoxviruses. Its epitope is localized on a tetrapeptide located at the VACV homologue A27 (C-terminal epitope #5 (aa 68-71). Ectromelia virus (ECTV) is a virus of the family Poxviridae and the genus Orthopoxvirus that causes mousepox, a disease of mice. Mousepox causes skin lesions and generalized disease, which can be fatal. Orthopoxvirus has been associated with smallpox, cowpox, horsepox and monkeypox.
Immunogen
Sucrose-gradient purified Ectromelia virus (ECTV) Strain Munich 1
Antibody Isotype
Mouse IgG2a/Human IgG1, Kappa
Clone
MP-X11
Clonality
Monoclonal Antibody
Purification
Protein A affinity purified
Applications
Immunogold electron microscopy; IP; WB; ELISA; FC; IF
Application Notes
This antibody is recommended for the detection and analysis of Orthopox viruses, especially Ectromelia virus (ECTV). It binds to the epitope on the virus which seems not to be engaged with viral cell fusion. VACV-5B1 (5B1/2G6) is a non-neutralizing clone that can be utilized for the detection of the analog of A27 protein on Orthopoxviruses. This antibody's antigenic site is located in a functionally very important areawithin the C-terminus of the A27 protein; in this hydrophilic region, the two cysteines at positions71 and 72 are responsible for formation of disulfide bonds and, therefore, play an important role for a functionally active trimeric A27 structure. Mouse IgG2a version of this clone (EM-5B1/2G6) was also used to develop an ELISA capture assay as a capture antibody and was shown to be superior in detecting Orthopox virues in comparison with other monoclonal and polyclonal clones. It makes it a very good candidate for the development of an anti-orthopox viruse detection assay.
Buffer
PBS with 0.02% Proclin 300
Preservative
0.02% Proclin 300
Storage
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Target
Target
A27
Introduction
Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It causes a disease that has similar charactersists to smallpox but with a milder rash and a lower death rate. The virus causes disease in both humans and animals.
Alternative Names
Orthopoxvirus; Monkeypox; Ectromelia virus; ECTV; ECTV-5B1/2G6; EM-5B1/2G6; Mab EX-uUl 5BA 05.11.20
Warnings
For Research Use Only. Not for Clinical Use or Diagnostic Use.

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