Anti-Vaccinia Virus A13 Monoclonal Antibody (MP-X8) (Mouse IgG2a/Human IgM, Kappa)(MPYF-0822-YF8)
This antibody recognizes Vaccinia Virus A13. The isotype is Mouse IgG2a/Human IgM, Kappa. It can be used in applications: Neut; SPR; WB; ELISA; IF.
Product Description
Species Reactivity
Vaccinia Virus
Cross Reactivity
Orthopoxvirus; Monkeypox Virus; Variola Virus
Specificity
This antibody binds the A13 membrane protein of the monkeypox virus and vaccinia virus. It recognizes an epitope comprising of a 10-amino acid sequence conserved in all orthopoxviruses, including viriola virus and monkeypox virus.
Immunogen
WR strain of live vaccinia virus (VACV)
Antibody Isotype
Mouse IgG2a/Human IgM, Kappa
Clonality
Monoclonal Antibody
Purification
Protein A affinity purified
Applications
Neut; SPR; WB; ELISA; IF
Application Notes
The original IgG2a version of this antibody bound to recombinant A13 protein expressed in E. coli, MBP conjugated VACV A13 in an ELISA. The original IgG2a antibody bound recombinant A13 proteins from E. coli with an affinity of 3.4 nM as detected by surface plasmon resonance. This antibody was also capable of recognizing a 12-kda protein in a Western blot of VACV-infected HeLa cells. This antibody stained viral factories and virion-size particles in immunofluorescence analysis of infected cells. This antibody was also reported to neutralized VACV mature virions in a plaque reduction assay. Passive immunization of mice with this antibody protected mice against intranasal VACV infection. This antibody generated against smallpox vaccine might contribute to immune protection against orthopoxviruses.
Buffer
PBS with 0.02% Proclin 300
Preservative
0.02% Proclin 300
Storage
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Reference
An epitope conserved in orthopoxvirus A13 envelope protein is the target of neutralizing and protective antibodies.
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Target
Introduction
Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It causes a disease that has similar charactersists to smallpox but with a milder rash and a lower death rate. The virus causes disease in both humans and animals. A13 is essential for the encapsidation of DNA into immature virions (IV) and the subsequent maturation of IV into mature virions (MV).
Alternative Names
VACV; monkeypox virus; Protein P8; A13L; vaccinia virus; smallpox virus; VACV virion membrane proteins; Virion membrane protein A13; VACWR132
Warnings
For Research Use Only. Not for Clinical Use or Diagnostic Use.
Datasheet